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Genom Data. 2015 Jun 20;5:284-91. doi: 10.1016/j.gdata.2015.06.018. eCollection 2015 Sep.

Genome analysis of rice-blast fungus Magnaporthe oryzae field isolates from southern India.

Author information

1
Genomics Laboratory, Centre for Cellular and Molecular Platforms, Bangalore 560065, India.
2
Genomics Laboratory, Centre for Cellular and Molecular Platforms, Bangalore 560065, India ; Marker Assisted Selection Laboratory, Department of Genetics and Plant Breeding, University of Agricultural Sciences, Bangalore, India.
3
Centre for Genome Research, Department of Microbiology and Biotechnology Centre, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara 390002, India.

Abstract

The Indian subcontinent is the center of origin and diversity for rice (Oryza sativa L.). The O. sativa ssp. indica is a major food crop grown in India, which occupies the first and second position in area and production, respectively. Blast disease caused by Magnaporthe oryzae is a major constraint to rice production. Here, we report the analysis of genome architecture and sequence variation of two field isolates, B157 and MG01, of the blast fungus from southern India. The 40 Mb genome of B157 and 43 Mb genome of MG01 contained 11,344 and 11,733 predicted genes, respectively. Genomic comparisons unveiled a large set of SNPs and several isolate specific genes in the Indian blast isolates. Avr genes were analyzed in several sequenced Magnaporthe strains; this analysis revealed the presence of Avr-Pizt and Avr-Ace1 genes in all the sequenced isolates. Availability of whole genomes of field isolates from India will contribute to global efforts to understand genetic diversity of M. oryzae population and to track the emergence of virulent pathotypes.

KEYWORDS:

Genome comparison; Isolate specific genes; Magnaporthe; Next generation sequencing; Single nucleotide polymorphism

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