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Genom Data. 2015 Apr 8;4:96-8. doi: 10.1016/j.gdata.2015.03.015. eCollection 2015 Jun.

Profiling of embryonic nuclear vs. cellular RNA in Arabidopsis thaliana.

Author information

1
Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.
2
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany.
3
Department of Cell Biology, Max Planck Institute for Developmental Biology, Tübingen 72076, Germany ; Department of Developmental Genetics, Center for Plant Molecular Biology, University of Tübingen, Tübingen 72076, Germany.

Abstract

In Arabidopsis, various cell type-specific whole-genome expression analyses have been conducted. However, the vast majority of these were performed with cellular RNA from root tissues or other easily accessible cell types [1]. Nuclear RNA was neglected for a long time as not being representative for transcriptomic studies. In recent years, however, there have been reports describing the validity of nuclear RNA for these types of studies [2,3]. Here we describe the generation, quality assessment and analysis of nuclear transcriptomic data from Arabidopsis embryos published by Slane et al. (2014) [4]. Comparison of nuclear with cellular gene expression demonstrated the usefulness of nuclear transcriptomics.

KEYWORDS:

Arabidopsis thaliana; Gene expression; Microarray; Nuclear and cellular transcriptome; Pro-embryo and suspensor transcriptome

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