The HER2 amplicon includes several genes required for the growth and survival of HER2 positive breast cancer cells - A data description

Vesa Hongisto; Miriam Ragle Aure; Rami Mäkelä; Kristine Kleivi Sahlberg

doi:10.1016/j.gdata.2014.06.025

The HER2 amplicon includes several genes required for the growth and survival of HER2 positive breast cancer cells - A data description

Genom Data. 2014 Jul 22:2:249-53. doi: 10.1016/j.gdata.2014.06.025. eCollection 2014 Dec.

Authors

Vesa Hongisto¹, Miriam Ragle Aure², Rami Mäkelä¹, Kristine Kleivi Sahlberg³

Affiliations

¹ VTT Technical Research Centre of Finland, Turku, Finland.
² K.G. Jebsen Centre of Breast Cancer, Department of Genetics, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.
³ K.G. Jebsen Centre of Breast Cancer, Department of Genetics, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway ; Department of Research, VestreViken, Drammen, Norway.

Abstract

A large number of breast cancers are characterized by amplification and overexpression of the chromosome segment surrounding the HER2 (ERBB2) oncogene. As the HER2 amplicon at 17q12 contains multiple genes, we have systematically explored the role of the HER2 co-amplified genes in breast cancer cell growth and their relation to trastuzumab resistance. We integrated array comparative genomic hybridization (aCGH) data of the HER2 amplicon from 71 HER2 positive breast tumors and 10 cell lines with systematic functional RNA interference analysis of 23 core amplicon genes with several phenotypic endpoints in a panel of trastuzumab responding and non-responding HER2 positive breast cancer cells. In this Data in Brief we give a detailed description of the experimental procedures and the data analysis methods used in the study (1).

Keywords: Drug treatment; HER2 positive breast cancer; Lysate microarrays; SiRNA; aCGH.