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G3 (Bethesda). 2015 Oct 19;5(12):2775-82. doi: 10.1534/g3.115.022608.

Influences of LIN-12/Notch and POP-1/TCF on the Robustness of Ventral Uterine Cell Fate Specification in Caenorhabditis elegans Gonadogenesis.

Author information

1
Department of Genetics and Development, Columbia University Medical Center, New York 10032.
2
Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York 10032.
3
Department of Genetics and Development, Columbia University Medical Center, New York 10032 Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York 10032 iva.columbia@gmail.com.

Abstract

The prospective ventral uterus of the hermaphrodite gonad primordium consists of two pairs of sister cells, with each pair consisting of a proximal "α" cell and a distal "β" cell. All four cells initially are competent to become the anchor cell (AC), a unique cell type that acts as the organizer of subsequent uterine and vulval development. However, the β cells soon lose this competence and always become ventral uterine precursor cells (VUs), whereas the α cells maintain their AC competence longer, until lin-12/Notch-mediated interactions between them specify one as the AC and the other as a VU. Here, we investigate this asymmetry in developmental potential and VU fate specification between the α and β sister cells. We find evidence that lin-12 activity contributes to the robustness of βVU fate at elevated temperature, that the Caenorhabditis elegans Notch paralog glp-1 is not functionally redundant with lin-12 in specifying βVU fate, and that the activity of POP-1, the sole C. elegans TCF ortholog, influences βVU fate. We propose a model for how Wnt and LIN-12/Notch signaling together lead to robust specification of the βVU fate.

KEYWORDS:

C. elegans; Notch; POP-1; gonadogenesis; lin-12

PMID:
26483009
PMCID:
PMC4683648
DOI:
10.1534/g3.115.022608
[Indexed for MEDLINE]
Free PMC Article

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