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Pharmacol Rep. 2015 Dec;67(6):1168-77. doi: 10.1016/j.pharep.2015.04.013. Epub 2015 May 11.

Chelidonine, a principal isoquinoline alkaloid of Chelidonium majus, attenuates eosinophilic airway inflammation by suppressing IL-4 and eotaxin-2 expression in asthmatic mice.

Author information

1
Institute of Traditional Medicine & Bioscience, Daejeon University, Daejeon, Republic of Korea.
2
Department of Herbology, College of Korean Medicine, Sangji University, Wonju, Republic of Korea.
3
Department of Herbology, College of Korean Medicine, Sangji University, Wonju, Republic of Korea. Electronic address: lyc072@sangji.ac.kr.

Abstract

BACKGROUND:

Chelidonine, a major bioactive, isoquinoline alkaloid ingredient in Chelidonium majus, exhibits anti-inflammatory and other pharmacological properties. However, its molecular mechanisms in asthma remain unclear. In this work we investigated chelidonine's effect and mechanism in airway inflammation in a mouse model of allergic asthma.

METHODS:

The mice were sensitized to ovalbumin followed by aerosol allergen challenges and determination of chelidonine's effect on enhanced pause (Penh), pulmonary eosinophilic infiltration, eotaxin-2, interleukin-4 (IL-4), IL-13, OVA-specific IgE production, and several transcription factors.

RESULT:

Chelidonine strongly suppressed airway eosinophilia, expression of eotaxin-2, IL-4, and IL-13 cytokine production in bronchoalveolar lavage fluid (BALF). It also attenuated lung IL-17, and eotaxin-2 mRNA expression levels. Moreover, it suppressed eotaxin-2 and IL-17 production in accordance with up- and downregulation of forkhead box p3 (Foxp3), and signal transducer and activator of transcription (STAT6) expression, respectively.

CONCLUSIONS:

Chelidonine has profound inhibitory effects on airway inflammation and this effect is caused by suppression of IL-4, eotaxin-2, and OVA-specific IgE production through the STAT6 and Foxp3 pathways. So chelidonine can improve allergic asthma in mice and be a novel anti-asthma therapeutic.

KEYWORDS:

Asthma; Chelidonine; Eotaxin-2; Foxp3; STAT6

PMID:
26481537
DOI:
10.1016/j.pharep.2015.04.013
[Indexed for MEDLINE]

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