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Fish Shellfish Immunol. 2016 Jul;54:144-52. doi: 10.1016/j.fsi.2015.10.019. Epub 2015 Oct 19.

Identification and characterization of a mitochondrial unfolded protein response transcription factor ATFS-1 in Litopenaeus vannamei.

Author information

1
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
2
School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
3
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China.
4
Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China; State Key Laboratory for Biocontrol, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, 510275, PR China. Electronic address: chenyh6@mail.sysu.edu.cn.

Abstract

A mitochondrial specific stress response termed mitochondrial unfolded protein response (UPR(mt)) is activated in responding to disturbance of protein homeostasis in mitochondria. The activating transcription factor associated with stress-1 (designated as ATFS-1) is the key regulator of UPR(mt). To investigating the roles of ATFS-1 (LvATFS-1) in Litopenaeus vannamei mitochondrial stress remission and immunity, it's full length cDNA was cloned. The open reading frame of LvATFS-1 was 1, 557 bp in length, deducing to a 268 amino acids protein. LvATFS-1 was highly expressed in muscle, hemocytes and eyestalk. Subcellular location assays showed that N-terminal of LvATFS-1 contained a mitochondrial targeting sequence, which could directed the fused EGFP located to mitochondria. And the C-terminal of LvATFS-1, which had a nuclear localization signal, expressed in nucleus. The in vitro experiments verified that LvATFS-1 could reduced the level of intracellular reactive oxygen species (ROS). And results of real-time RT-PCR indicated that LvATFS-1 might scavenge excess ROS via ROS-eliminating genes regulation. Reporter gene assays showed that LvATFS-1 could upregulated the expression of the antimicrobial peptide genes in Drosophila Schneider 2 cells. Results of real-time RT-PCR showed that Vibrio alginolyticus or white spot syndrome virus (WSSV) infection induced the expression of LvATFS-1. And knocked-down LvATFS-1 by RNAi resulted in a higher cumulative mortality of L. vannamei upon V. alginolyticus or WSSV infection. These results suggested that LvATFS-1 not only rolled in mitochondrial specific stress responding, but also important for L. vannamei immunologic defence.

KEYWORDS:

ATFS-1; Innate immunity; Litopenaeus vannamei; Mitochondrial unfolded protein response; ROS

PMID:
26481519
DOI:
10.1016/j.fsi.2015.10.019
[Indexed for MEDLINE]

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