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ASAIO J. 2016 Jan-Feb;62(1):100-5. doi: 10.1097/MAT.0000000000000296.

Early Results of Novel Bovine Pericardial Patch Using Comprehensive Anticalcification Procedure in a Swine Model.

Author information

1
From the *Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul, South Korea; †Cardiovascular Center, Sejong General Hospital, Bucheon, South Korea; ‡Seoul National University Bundang Hospital, Seongnam, South Korea; and §SMG-SNU Boramae Medical Center, Seoul, South Korea.

Abstract

We evaluated the short-term safety and effectiveness of our comprehensive anticalcification procedure in swine model. Our comprehensive anticalcification procedure consisted of four steps, including decellularization with sodium dodecyl sulfate and tritonX-100, space filler treatment with polyethylene glycol (PEG), glutaraldehyde cross-linking with organic solvent, and detoxification with glycine. We simultaneously implanted both the commercially available bovine pericardial patch (Supple Peri-Guard) and novel bovine pericardial patch processed by the comprehensive anticalcification procedure into the main pulmonary artery in seven pigs. Every pig underwent a cardiac angiography and was killed on the postoperative day 28. The extracted patches were stained with hematoxylin and eosin. All pigs survived for 4 weeks without any complication. Cardiac angiography showed the absence of leakage and structural problem. Neointimas were formed evenly without intimal hyperplasia. There were no significant differences in the degree of inflammation, necrosis, and calcification between the novel and commercially available patch (p = 0.450, p = 0.317, p = 0.999). Novel bovine pericardial patch using comprehensive anticalcification procedure was similar to existing cardiovascular patch in early surgical results in a swine model. The comprehensive anticalcification procedure could facilitate appropriate bioprosthetic properties of the bovine pericardium.

PMID:
26479466
DOI:
10.1097/MAT.0000000000000296
[Indexed for MEDLINE]

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