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Nat Struct Mol Biol. 2015 Nov;22(11):920-3. doi: 10.1038/nsmb.3111. Epub 2015 Oct 19.

Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.

Author information

1
Laboratory of Protein and Nucleic Acid Chemistry, Rockefeller University, New York, New York, USA.
2
Tri-Institutional Training Program in Chemical Biology, Rockefeller University, New York, New York, USA.
3
Proteomics Resource Center, Rockefeller University, New York, New York, USA.

Abstract

Eukaryotic ribosome biogenesis involves a plethora of ribosome-assembly factors, and their temporal order of association with preribosomal RNA is largely unknown. By using Saccharomyces cerevisiae as a model organism, we developed a system that recapitulates and arrests ribosome assembly at early stages, thus providing in vivo snapshots of nascent preribosomal particles. Here we report the stage-specific order in which 70 ribosome-assembly factors associate with preribosomal RNA domains, thereby forming the 6-MDa small-subunit processome.

PMID:
26479197
DOI:
10.1038/nsmb.3111
[Indexed for MEDLINE]

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