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Nat Biotechnol. 2015 Nov;33(11):1162-4. doi: 10.1038/nbt.3389. Epub 2015 Oct 19.

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins.

Author information

1
Convergence Research Center for Functional Plant Products, Advanced Institutes of Convergence Technology, Yeongtong-gu, Suwon-si, Gyeonggi-do, Korea.
2
Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea.
3
Department of Chemistry, Seoul National University, Seoul, South Korea.
4
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, South Korea.
5
Plant Genomics and Breeding Institute, Seoul National University, Seoul, South Korea.

Abstract

Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.

PMID:
26479191
DOI:
10.1038/nbt.3389
[Indexed for MEDLINE]

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