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ACS Appl Mater Interfaces. 2015 Nov 11;7(44):24733-40. doi: 10.1021/acsami.5b07453. Epub 2015 Oct 27.

Colloidal Gold--Collagen Protein Core--Shell Nanoconjugate: One-Step Biomimetic Synthesis, Layer-by-Layer Assembled Film, and Controlled Cell Growth.

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State Key Laboratory of Metastable Materials Science and Technology, Yanshan University , Qinhuangdao 066004, China.
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University , Qinhuangdao 066004, China.
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences , Beijing 100190, China.
Institute of Advanced Materials, Jiangsu University , Zhenjiang 212013, Jiangsu, China.
National Center for Nanoscience and Technology , Beijing 100190, China.
Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, D-14476 Potsdam/Golm, Germany.


The biogenic synthesis of biomolecule-gold nanoconjugates is of key importance for a broad range of biomedical applications. In this work, a one-step, green, and condition-gentle strategy is presented to synthesize stable colloidal gold-collagen core-shell nanoconjugates in an aqueous solution at room temperature, without use of any reducing agents and stabilizing agents. It is discovered that electrostatic binding between gold ions and collagen proteins and concomitant in situ reduction by hydroxyproline residues are critically responsible for the formation of the core-shell nanoconjugates. The film formed by layer-by-layer assembly of such colloidal gold-collagen nanoconjugates can notably improve the mechanical properties and promote cell adhesion, growth, and differentiation. Thus, the colloidal gold-collagen nanoconjugates synthesized by such a straightforward and clean manner, analogous to a biomineralization pathway, provide new alternatives for developing biologically based hybrid biomaterials toward a range of therapeutic and diagnostic applications.


cell adhesion and growth; collagen protein; colloidal gold nanoparticles; core−shell nanoconjugates; layer-by-layer assembly

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