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Methods. 2016 Mar 1;96:69-74. doi: 10.1016/j.ymeth.2015.10.005. Epub 2015 Oct 17.

TransgeneOmics--A transgenic platform for protein localization based function exploration.

Author information

1
Max Planck Institute of Molecular Cell Biology and Genetics Dresden, Germany.

Abstract

The localization of a protein is intrinsically linked to its role in the structural and functional organization of the cell. Advances in transgenic technology have streamlined the use of protein localization as a function discovery tool. Here we review the use of large genomic DNA constructs such as bacterial artificial chromosomes as a transgenic platform for systematic tag-based protein function exploration.

KEYWORDS:

BAC transgenesis; Genome engineering; Protein localization; Protein tagging; Recombineering

PMID:
26475212
DOI:
10.1016/j.ymeth.2015.10.005
[Indexed for MEDLINE]
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