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J Evid Based Complementary Altern Med. 2016 Oct;21(4):NP18-24. doi: 10.1177/2156587215611517. Epub 2015 Oct 16.

Immunomodulatory Effects of Psyllium Extract on Helicobacter pylori Interaction With Gastric Epithelial Cells.

Author information

1
Aga Khan University, Karachi, Pakistan javed.yakoob@aku.edu.
2
Aga Khan University, Karachi, Pakistan.

Abstract

Natural plant product Psyllium has anti-inflammatory activity that can modulate the function of cytokines. We determined the effect of Psyllium husk extract on interleukin (IL)-8 and NF-κB secretion by gastric epithelial cells in response to Helicobacter pylori Human gastric adenocarcinoma cell line (AGS) cells were pretreated with Psyllium extract in different concentrations before H pylori infection. Cell culture supernatant was analyzed for IL-8 and NF-κB by ELISA. RNA from cells was used for real-time polymerase chain reaction for messenger RNA expression of IL-8. Psyllium extract 5 and 10 μg/mL markedly (P < .001) lowered basal IL-8 by 64.71% and 74.51%, respectively, and H pylori-stimulated IL-8 was also (P < .001) lowered by 41.67% and 66.67%, respectively. Psyllium 5 and 10 μg/mL also reduced (P < .0001) cagA-positive H pylori-induced IL-8 mRNA expression by 42.3% and 67.6%, respectively. Psyllium also reduced (P = .0001) NF-κB in response to H pylori strains confirming its role as an anti-inflammatory agent.

KEYWORDS:

Helicobacter pylori; NF-κB; gastric epithelial cells; interleukin-8; psyllium

PMID:
26474925
DOI:
10.1177/2156587215611517
[Indexed for MEDLINE]

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