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Viruses. 2015 Oct 13;7(10):5319-27. doi: 10.3390/v7102873.

Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses.

Author information

1
Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. vgtran@wisc.edu.
2
Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA. vgtran@wisc.edu.
3
Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. dspoole@wisc.edu.
4
Carbone Cancer Center, University of Wisconsin Madison, Madison, WI 53706, USA. jjjeffery@wisc.edu.
5
GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. sheahan@email.unc.edu.
6
GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. donald.r.creech@gsk.com.
7
PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA. Aleksey.Yevtodiyenko@perkinelmer.com.
8
GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. andy.j.peat@gsk.com.
9
PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA. Kevin.Francis@perkinelmer.com.
10
GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA. shihyun.k.you@gsk.com.
11
Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA. amehle@wisc.edu.

Abstract

Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography-computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.

KEYWORDS:

influenza virus; multi-modal imaging; multiplicity reactivation; reporter virus

PMID:
26473913
PMCID:
PMC4632381
DOI:
10.3390/v7102873
[Indexed for MEDLINE]
Free PMC Article

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