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Food Chem. 2016 Mar 1;194:920-7. doi: 10.1016/j.foodchem.2015.08.094. Epub 2015 Aug 24.

Antioxidant and anti-ageing activities of citrus-based juice mixture.

Author information

1
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea.
2
EBO Co., LTD., Cheongju 361-748, Republic of Korea.
3
Department of Biology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
4
Hurum Central Research Institute, Hurum Co., Ltd., Ochang 363-880, Republic of Korea.
5
Hurum Central Research Institute, Hurum Co., Ltd., Ochang 363-880, Republic of Korea. Electronic address: dusvnd608@hurum.co.kr.
6
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: loh99@kangwon.ac.kr.

Abstract

The production of excessive reactive oxygen species by exposure to oxidative stress and solar radiation are primary factors in skin damage. We examined the effects of a citrus-based juice mixture and its bioactive compounds on antioxidant and anti-ageing activities in human dermal fibroblasts and hairless mice via the regulation of antioxidant enzymes and the mitogen-activated protein kinase pathway. The citrus-based juice mixture reduced H2O2-induced cell damage and intracellular reactive oxygen species production in human dermal fibroblasts. Citrus-based juice mixture pretreatment suppressed the activation of the H2O2-mediated mitogen-activated protein kinase pathway by activating the expression of activator protein 1 and matrix metalloproteinases. Moreover, it increased the expression levels of antioxidant enzymes such as glutathione reductase, catalase and manganese superoxide dismutase. In addition, oral administration of the citrus-based juice mixture decreased skin thickness and wrinkle formation and increased collagen content on an ultraviolet light B-exposed hairless mouse. These results indicate that the citrus-based juice mixture is a potentially healthy beverage for the prevention of oxidative stress-induced premature skin ageing.

KEYWORDS:

Anti-ageing; Antioxidant; Citrus-based juice mixture; Hairless mice; Human dermal fibroblasts

PMID:
26471635
DOI:
10.1016/j.foodchem.2015.08.094
[Indexed for MEDLINE]

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