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Food Chem. 2016 Mar 1;194:555-60. doi: 10.1016/j.foodchem.2015.08.049. Epub 2015 Aug 14.

Simple column-switching ion chromatography method for determining eight monosaccharides and oligosaccharides in honeydew and nectar.

Author information

1
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Hangzhou 310007, China.
2
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China. Electronic address: wnn8511@163.com.
3
Department of Chemistry, Lishui University, Lishui 323000, China.
4
School of Pharmaceutical and Chemical Engineering, Taizhou University, Taizhou 317000,China.
5
Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310007, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Hangzhou 310007, China. Electronic address: zhuyan@zju.edu.cn.

Abstract

Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity (r⩾0.9994) and afforded low limits of detection ranging from 1.55 to 10.17μgL(-1) for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% (n=6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar.

KEYWORDS:

Column-switching; Honeydew; Ion chromatography; Nectar; Sugars

PMID:
26471592
DOI:
10.1016/j.foodchem.2015.08.049
[Indexed for MEDLINE]

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