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Biochem Biophys Res Commun. 2015 Nov 27;467(4):928-34. doi: 10.1016/j.bbrc.2015.10.041. Epub 2015 Oct 16.

Camphene isolated from essential oil of Piper cernuum (Piperaceae) induces intrinsic apoptosis in melanoma cells and displays antitumor activity in vivo.

Author information

1
Experimental Oncology Unit (UNONEX), Federal University of São Paulo, SP, Brazil. Electronic address: nataliagirola@hotmail.com.
2
Experimental Oncology Unit (UNONEX), Federal University of São Paulo, SP, Brazil.
3
Laboratory of Tumor Immunology, Institute of Biomedical Sciences, University of São Paulo, SP, Brazil.
4
Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, SP, Brazil.
5
Institute of Biosciences, University of São Paulo, SP, Brazil.
6
Interdepartmental Group of Health Economics (Grides), Federal University of São Paulo, SP, Brazil.

Abstract

Natural monoterpenes were isolated from the essential oil of Piper cernuum Vell. (Piperaceae) leaves. The crude oil and the individual monoterpenes were tested for cytotoxicity in human tumor cell lineages and B16F10-Nex2 murine melanoma cells. In the present work we demonstrate the activity of camphene against different cancer cells, with its mechanism of action being investigated in vitro and in vivo in murine melanoma. Camphene induced apoptosis by the intrinsic pathway in melanoma cells mainly by causing endoplasmic reticulum (ER) stress, with release of Ca(2+) together with HmgB1 and calreticulin, loss of mitochondrial membrane potential and up regulation of caspase-3 activity. Importantly, camphene exerted antitumor activity in vivo by inhibiting subcutaneous tumor growth of highly aggressive melanoma cells in a syngeneic model, suggesting a promising role of this compound in cancer therapy.

KEYWORDS:

Antitumor; Apoptosis; Camphene; Melanoma

PMID:
26471302
DOI:
10.1016/j.bbrc.2015.10.041
[Indexed for MEDLINE]

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