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Biochem Biophys Res Commun. 2015 Nov 27;467(4):935-40. doi: 10.1016/j.bbrc.2015.10.040. Epub 2015 Oct 19.

Overexpressed human heme Oxygenase-1 decreases adipogenesis in pigs and porcine adipose-derived stem cells.

Author information

1
Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: ejpch04@snu.ac.kr.
2
Samsung Biomedical Research Institute, SAIT, SEC, Suwon 16419, Republic of Korea. Electronic address: okjae.koo@gmail.com.
3
Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: bclee@snu.ac.kr.

Abstract

Adipose-derived mesenchymal stem cells (ADSC) are multipotent, which means they are able to differentiate into several lineages in vivo and in vitro under proper conditions. This indicates it is possible to determine the direction of differentiation of ADSC by controlling the microenvironment. Heme oxygenase 1 (HO-1), a type of antioxidant enzyme, attenuates adipogenicity and obesity. We produced transgenic pigs overexpressing human HO-1 (hHO-1-Tg), and found that these animals have little fatty tissue when autopsied. To determine whether overexpressed human HO-1 suppresses adipogenesis in pigs, we analyzed body weight increases of hHO-1-Tg pigs and wild type (WT) pigs of the same strain, and induced adipogenic differentiation of ADSC derived from WT and hHO-1-Tg pigs. The hHO-1-Tg pigs had lower body weights than WT pigs from 16 weeks of age until they died. In addition, hHO-1-Tg ADSC showed reduced adipogenic differentiation and expression of adipogenic molecular markers such as PPARγ and C/EBPα compared to WT ADSC. These results suggest that HO-1 overexpression reduces adipogenesis both in vivo and in vitro, which could support identification of therapeutic targets of obesity and related metabolic diseases.

KEYWORDS:

ADSC; Adipogenic differentiation; C/EBPα; HO-1; PPARγ

PMID:
26471299
DOI:
10.1016/j.bbrc.2015.10.040
[Indexed for MEDLINE]

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