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Adv Clin Exp Med. 2015 May-Jun;24(3):447-51. doi: 10.17219/acem/29223.

An Assay of Selected Serum Amino Acids in Patients with Type 2 Diabetes Mellitus.

Author information

1
Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Czech Republic.
2
Department of Clinical Biochemistry and Diagnostics, Faculty of Chemical Technology, Regional Hospital Pardubice, Czech Republic.

Abstract

BACKGROUND:

Amino acids are the building blocks of proteins. In case of insulin resistance, which is typical for type 2 diabetes mellitus (T2DM), proteolysis is increased and protein synthesis is decreased; therefore, we can observe changes in the levels of amino acids in diabetics vs. non-diabetics.

OBJECTIVES:

The aim of this study was to find differences in the levels of selected amino acids between patients with diabetes (type 2) and a control group.

MATERIAL AND METHODS:

Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde in the presence of potassium cyanide to form fluorescent 1-cyanobenz(f)isoindole product. Amino acids derivatives were measured using a high-performance liquid chromatography with fluorescence detection. The serum levels of glucose were determined using an automatic biochemistry analyzer, glycated hemoglobin HbA1c was measured by cation exchange chromatography.

RESULTS:

A total of 19 serum amino acids in T2DM patients and non-diabetics were measured. There were 9 amino acids, which were significantly different in these groups (p<0.05). Significantly decreased levels of arginine, asparagine, glycine, serine, threonine and significantly increased levels of alanine, isoleucine, leucine, valine in diabetics were found.

CONCLUSIONS:

Significant difference in metabolism of amino acids between diabetics and non-diabetics were observed. The altered levels of amino acids in diabetic patients could be a suitable predictor of diabetes.

PMID:
26467133
[Indexed for MEDLINE]
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