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Adv Clin Exp Med. 2015 May-Jun;24(3):447-51. doi: 10.17219/acem/29223.

An Assay of Selected Serum Amino Acids in Patients with Type 2 Diabetes Mellitus.

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Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Czech Republic.
Department of Clinical Biochemistry and Diagnostics, Faculty of Chemical Technology, Regional Hospital Pardubice, Czech Republic.



Amino acids are the building blocks of proteins. In case of insulin resistance, which is typical for type 2 diabetes mellitus (T2DM), proteolysis is increased and protein synthesis is decreased; therefore, we can observe changes in the levels of amino acids in diabetics vs. non-diabetics.


The aim of this study was to find differences in the levels of selected amino acids between patients with diabetes (type 2) and a control group.


Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde in the presence of potassium cyanide to form fluorescent 1-cyanobenz(f)isoindole product. Amino acids derivatives were measured using a high-performance liquid chromatography with fluorescence detection. The serum levels of glucose were determined using an automatic biochemistry analyzer, glycated hemoglobin HbA1c was measured by cation exchange chromatography.


A total of 19 serum amino acids in T2DM patients and non-diabetics were measured. There were 9 amino acids, which were significantly different in these groups (p<0.05). Significantly decreased levels of arginine, asparagine, glycine, serine, threonine and significantly increased levels of alanine, isoleucine, leucine, valine in diabetics were found.


Significant difference in metabolism of amino acids between diabetics and non-diabetics were observed. The altered levels of amino acids in diabetic patients could be a suitable predictor of diabetes.

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