Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2015 Oct 27;112(43):13378-83. doi: 10.1073/pnas.1510854112. Epub 2015 Oct 14.

Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm.

Author information

1
Plant Gene Expression Center, US Department of Agriculture-Agricultural Research Service, Albany, CA 94710; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720; Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden; Linnean Center of Plant Biology, Uppsala, 750 07 Uppsala, Sweden;
2
Plant Genomics Laboratory, Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal.
3
Plant Gene Expression Center, US Department of Agriculture-Agricultural Research Service, Albany, CA 94710; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720;
4
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden; Linnean Center of Plant Biology, Uppsala, 750 07 Uppsala, Sweden; sheilamc@berkeley.edu claudia.kohler@slu.se.
5
Plant Gene Expression Center, US Department of Agriculture-Agricultural Research Service, Albany, CA 94710; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720; sheilamc@berkeley.edu claudia.kohler@slu.se.

Abstract

An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.

KEYWORDS:

mRNA transport; pollen; protein phosphatase 2C; sperm; vegetative nucleus

PMID:
26466609
PMCID:
PMC4629363
DOI:
10.1073/pnas.1510854112
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center