Format

Send to

Choose Destination
Stand Genomic Sci. 2015 May 1;10:12. doi: 10.1186/1944-3277-10-12. eCollection 2015.

Draft genome sequence of Mesotoga strain PhosAC3, a mesophilic member of the bacterial order Thermotogales, isolated from a digestor treating phosphogypsum in Tunisia.

Author information

1
Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, F-13288 Marseille, cedex 09, France ; Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Faculté des Sciences de Carthage, Centre Urbain Nord, BP 676, Tunis, 1080 Tunisia.
2
CNRS, UMR 5558, Laboratoire de Biométrie et Biologie Evolutive, Université de Lyon, Université Lyon 1, 43 boulevard du 11 novembre 1918, Villeurbanne, F-69622 France.
3
Protéus SA, 70 Allée Graham Bell, Nîmes, F-30035 France.
4
Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, F-13288 Marseille, cedex 09, France.
5
Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Faculté des Sciences de Carthage, Centre Urbain Nord, BP 676, Tunis, 1080 Tunisia.
6
Aix-Marseille Université, CNRS, LCB-UMR7283, Marseille, F-13009 France.

Abstract

Mesotoga strain PhosAc3 was the first mesophilic cultivated member of the order Thermotogales. This genus currently contain two described species, M. prima and M. infera. Strain PhosAc3, isolated from a Tunisian digestor treating phosphogypsum, is phylogenetically closely related to M. prima strain MesG1.Ag.4.2(T). Strain PhosAc3 has a genome of 3.1 Mb with a G+C content of 45.2%. It contains 3,051 protein-coding genes of which 74.6% have their best reciprocal BLAST hit in the genome of the type species, strain MesG1.Ag.4.2(T). For this reason we propose to assign strain PhosAc3 as a novel ecotype of the Mesotoga prima species. However, in contrast with the M. prima type strain, (i) it does not ferment sugars but uses them only in the presence of elemental sulfur as terminal electron acceptor, (ii) it produces only acetate and CO2 from sugars, whereas strain MesG1.Ag.4.2(T) produces acetate, butyrate, isobutyrate, isovalerate, 2-methyl-butyrate and (iii) sulfides are also end products of the elemental sulfur reduction in theses growth conditions.

KEYWORDS:

Anaerobic; Mesophilic; Mesotoga; Thermotogales

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center