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Biomed Pharmacother. 2015 Oct;75:51-7. doi: 10.1016/j.biopha.2015.08.010. Epub 2015 Sep 2.

miR-425 inhibits melanoma metastasis through repression of PI3K-Akt pathway by targeting IGF-1.

Author information

1
Department of Plastic Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.
2
Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China.
3
Department of Plastic Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China. Electronic address: hu_zhensheng@126.com.

Abstract

miR-425 is a potential tumor suppressor in cancer, but its role in melanoma is still unknown. We aim to investigate miR-425 expression in melanoma tissues and cell lines. Next, cell proliferation, cell cycle, apoptosis and metastasis will be studied using lentivirus-mediated gain-of-function studies. The predicted results are stable miR-425 inhibits cell proliferation and metastasis and induced cell apoptosis. It is predicted that IGF-1 is a potential target gene of miR-495 by bioinformatics analysis. Then luciferase assay analysis identifies IGF-1 as a new direct target gene of miR-425 and miR-425 inhibits melanoma cancer progression via IGF-1. Collectively, our findings suggested that miR-425 may function as a tumor suppressor in melanoma by targeting IGF-1.

KEYWORDS:

IGF-1; Melanoma; Metastasis; miR-425

PMID:
26463631
DOI:
10.1016/j.biopha.2015.08.010
[Indexed for MEDLINE]

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