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J Clin Periodontol. 2015 Dec;42(12):1074-82. doi: 10.1111/jcpe.12470. Epub 2015 Nov 29.

Relationship between periodontitis-associated subgingival microbiota and clinical inflammation by 16S pyrosequencing.

Author information

1
FISABIO Foundation, Centre for Advanced Research in Public Health, Valencia, Spain.
2
Special Needs Unit and Periodontology Unit, Oral Sciences Research Group, School of Medicine and Dentistry, University of Santiago de Compostela, Santiago de Compostela, Spain.

Abstract

AIM:

To analyse the relationship between the chronic periodontitis-associated subgingival microbiota and clinical inflammation.

MATERIAL AND METHODS:

Sixty subjects with generalized chronic periodontitis participated in this study. Patients were divided into two groups according to their bleeding on probing (BOP) scores: BOP-1 group (mean scores ≤50% in sampled sites) and BOP-2 group (mean scores >50%). Subgingival bacterial samples from periodontal patients were studied by pyrosequencing PCR products of the 16S rRNA gene and by real-time PCR.

RESULTS:

In all the analysed subgingival samples, 102 bacterial genera and 203 species (from 41 genera of interest) were identified. Rarefaction curves showed a greater number of bacterial species in samples from BOP-2 group compared to BOP-1 group. The BOP-1 group had significantly higher abundance percentages of Anaeroglobus (especifically, A. geminatus), Capnocytophaga (especifically C. gingivalis), TM7 and Veillonella. The BOP-2 had significantly higher abundance percentages of Desulfobulbus (especially D. propionicus), Eubacterium (especially E. saphenum), Filifactor alocis, Streptococcus constellatus, Tannerella (especially, T. forsythia) and Treponema.

CONCLUSION:

16S pyrosequencing revealed that increased inflammation, at sites with periodontitis, is associated with a more diverse subgingival microbiota and specific changes in the bacterial composition, involving "established" periopathogens, symbionts and novel low-abundance pathobionts.

KEYWORDS:

16S ribosomal RNA; bacterial load; chronic periodontitis; microbiome; pyrosequencing; subgingival plaque

PMID:
26461079
DOI:
10.1111/jcpe.12470
[Indexed for MEDLINE]

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