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Int J Rheum Dis. 2016 Oct;19(10):968-973. doi: 10.1111/1756-185X.12783. Epub 2015 Oct 13.

Methylation and gene expression of histone deacetylases 6 in systemic lupus erythematosus.

Author information

1
Division of Geriatrics and Gerontology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
2
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
3
Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
4
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. jehsye@kmu.edu.tw.
5
Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. jehsye@kmu.edu.tw.
6
Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan. jehsye@kmu.edu.tw.
7
Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, Taiwan. jehsye@kmu.edu.tw.

Abstract

AIM:

The purpose of this study is to investigate the role of methylation in the histone deacetylases 6 (HDAC6) promoter and HDAC6 messenger RNA (mRNA) expression in the pathogenesis of systemic lupus erythematosus (SLE).

METHOD:

Direct bisulfite-polymerase chain reaction (PCR) sequencing was performed to detect the HDAC6 promoter methylation in 33 patients with SLE and 35 healthy controls. The HDAC6 mRNA expression was measured in 93 SLE patients and 84 healthy controls by using the method of quantitative real-time PCR.

RESULTS:

This study demonstrated that the methylation rates at HDAC6-680, -660 and -658 were significantly increased in the SLE patients compared with healthy controls (P = 0.041, 0.034 and 0.029, respectively). The SLE patients also had lower HDAC6 mRNA expression than the controls (P = 0.031). However, there was no significant difference in HDAC6 mRNA expression between patients with active and inactive SLE.

CONCLUSION:

The SLE patients had higher methylation in the HDAC6 promoter and lower HDAC6 mRNA expression than the controls. These changes may be related to the susceptibility of SLE. However, they are not associated with the disease activity of SLE.

KEYWORDS:

HDAC6; methylation; systemic lupus erythematosus

PMID:
26461065
DOI:
10.1111/1756-185X.12783
[Indexed for MEDLINE]

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