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Talanta. 2015 Dec 1;145:2-5. doi: 10.1016/j.talanta.2015.07.051. Epub 2015 Jul 17.

Paper-based ELISA to rapidly detect Escherichia coli.

Author information

1
Department of Orthopedic Surgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan; Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taiwan.
2
Department of Nephrology, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.
3
Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taiwan; Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 40705, Taiwan; Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
4
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
5
Department of Nephrology, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
6
Department of Orthopedic Surgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
7
Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
8
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan. Electronic address: chaomin@mx.nthu.edu.tw.

Abstract

Escherichia coli is a generic indicator of fecal contamination, and certain serotypes cause food- and water-borne illness such as O157:H7. In the clinic, detection of bacteriuria, which is often due to E. coli, is critical before certain surgical procedures or in cases of nosocomial infection to prevent further adverse events such as postoperative infection or sepsis. In low- and middle-income countries, where insufficient equipment and facilities preclude modern methods of detection, a simple, low-cost diagnostic device to detect E. coli in water and in the clinic will have significant impact. We have developed a simple paper-based colorimetric platform to detect E. coli contamination in 5h. On this platform, the mean color intensity for samples with 10(5)cells/mL is 0.118±0.002 (n=4), and 0.0145±0.003 (P<0.01⁎⁎) for uncontaminated samples. This technique is less time-consuming, easier to perform, and less expensive than conventional methods. Thus, paper-based ELISA is an innovative point-of-care diagnostic tool to rapidly detect E. coli, and possibly other pathogens when customized as appropriate, especially in areas that lack advanced clinical equipment.

KEYWORDS:

Escherichia coli; Paper-based ELISA; Point-of-care diagnostics; Urinary tract infection

PMID:
26459436
DOI:
10.1016/j.talanta.2015.07.051
[Indexed for MEDLINE]

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