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Methods Mol Biol. 2016;1349:25-34. doi: 10.1007/978-1-4939-3008-1_2.

Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination.

Author information

1
Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain.
2
Department of Biochemistry, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
3
Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS), University of Santiago de Compostela, Campus Vida, C/ Jenaro de la Fuente s/n, Santiago de Compostela, 15782, Spain. jose.martinez.costas@usc.es.

Abstract

Particulate material is more efficient in eliciting immune responses. Here we describe the production of microspheres formed by protein muNS-Mi from avian reoviruses, loaded with foreign epitopes by means of IC-Tagging, for their use as vaccines.

KEYWORDS:

Adjuvants; Avian reovirus; IC-Tagging; Microspheres; Particulate material; Vaccines

PMID:
26458827
DOI:
10.1007/978-1-4939-3008-1_2
[Indexed for MEDLINE]

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