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Biochimie. 2015 Dec;119:36-44. doi: 10.1016/j.biochi.2015.10.008. Epub 2015 Oct 9.

Poly(ADP-ribosyl)ation as a new posttranslational modification of YB-1.

Author information

1
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia.
2
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia.
3
Institute of Protein Research, RAS, Pushchino, Moscow Region, 142290, Russia; Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 829, Université d'Evry Val d'Essonne, Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, Evry, 91025, France.
4
Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 829, Université d'Evry Val d'Essonne, Laboratoire Structure-Activité des Biomolécules Normales et Pathologiques, Evry, 91025, France.
5
Institute of Protein Research, RAS, Pushchino, Moscow Region, 142290, Russia.
6
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk, 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia. Electronic address: lavrik@niboch.nsc.ru.

Abstract

Multifunctional Y-box binding protein 1 (YB-1) is actively studied as one of the components of cellular response to genotoxic stress. However, the precise role of YB-1 in the process of DNA repair is still obscure. In the present work we report for the first time new posttranslational modification of YB-1 - poly(ADP-ribosyl)ation, catalyzed by one of the main regulatory enzymes of DNA repair - poly(ADP-ribose)polymerase 1 (PARP1) in the presence of model DNA substrate carrying multiple DNA lesions. Therefore, poly(ADP-ribosyl)ation of YB-1 catalyzed with PARP1, can be stimulated by damaged DNA. The observed property of YB-1 underlines its ability to participate in the DNA repair by its involvement in the regulatory cascades of DNA repair.

KEYWORDS:

AP endonuclease 1 (APE1); Multiple DNA lesions; Poly(ADP-ribose) (PAR; Poly(ADP-ribose)polymerase 1 (PARP1); Y-box binding protein 1 (YB-1); pADPr)

PMID:
26453809
DOI:
10.1016/j.biochi.2015.10.008
[Indexed for MEDLINE]

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