Format

Send to

Choose Destination
Biochimie. 2015 Dec;119:16-26. doi: 10.1016/j.biochi.2015.10.004. Epub 2015 Oct 8.

MicroRNA-16 modulates macrophage polarization leading to improved insulin sensitivity in myoblasts.

Author information

1
Department of Biology, Dr. Reddy's Institute of Life Sciences, University of Hyderabad Campus, Hyderabad, Telangana, India.
2
Institute of Science, Nirma University, Sarkhej Gandhinagar Highway, Ahmedabad, Gujarat, India.
3
Department of Biology, Dr. Reddy's Institute of Life Sciences, University of Hyderabad Campus, Hyderabad, Telangana, India. Electronic address: kishorep@drils.org.

Abstract

Uncontrolled inflammation leads to several diseases such as insulin resistance, T2D and several types of cancers. The functional role of microRNAs in inflammation induced insulin resistance is poorly studied. MicroRNAs are post-transcriptional regulatory molecules which mediate diverse biological processes. We here show that miR-16 expression levels are down-regulated in different inflammatory conditions such as LPS/IFNγ or palmitate treated macrophages, palmitate exposed myoblasts and insulin responsive tissues of high sucrose diet induced insulin resistant rats. Importantly, forced expression of miR-16 in macrophages impaired the production of TNF-α, IL-6 and IFN-β leading to enhanced insulin stimulated glucose uptake in co-cultured skeletal myoblasts. Further, ectopic expression of miR-16 enhanced insulin stimulated glucose uptake in skeletal myoblasts via the up-regulation of GLUT4 and MEF2A, two key players involved in insulin stimulated glucose uptake. Collectively, our data highlight the important role of miR-16 in ameliorating inflammation induced insulin resistance.

KEYWORDS:

GLUT4; High sucrose diet; Inflammation; Insulin resistance; Macrophage polarization; microRNA

PMID:
26453808
DOI:
10.1016/j.biochi.2015.10.004
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center