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Pharm Biol. 2016;54(5):827-34. doi: 10.3109/13880209.2015.1087036. Epub 2015 Oct 9.

Effects of macamides on endurance capacity and anti-fatigue property in prolonged swimming mice.

Yang Q1,2, Jin W1,3,4, Lv X1, Dai P1,4, Ao Y1, Wu M1, Deng W1, Yu L1,3,4.

Author information

1
a Department of Biotechnology, Institute of Resource Biology and Biotechnology , College of Life Science and Technology, Huazhong University of Science and Technology , Wuhan , China .
2
b School of Physical Education and Sport , Central China Normal University , Wuhan , China .
3
c Key Laboratory of Molecular Biophysics , Ministry of Education , Wuhan , China , and.
4
d Wuhan Huashite Industrial Biotechnology Development Co. Ltd. , Wuhan Institute of Biotechnology , Wuhan , China.

Abstract

CONTEXT:

Lepidium meyenii Walp. (Brassicaceae), most commonly known as "maca", has been used as a food or folk medicine to improve vitality in Peru. Previous research demonstrated that lipid-soluble extract from maca improved swimming endurance capacity. Macamides are considered the typical lipid-soluble markers for maca and proved to have several pharmacological properties, such as improving sexual performance and neuroprotective activies.

OBJECTIVE:

The present study investigates the effects of macamides on endurance capacity and anti-fatigue property in prolonged swimming mice.

MATERIALS AND METHODS:

The Balb/c mice were divided into seven groups: a control group, low-dose groups of N-benzyllinoleamide, N-benzyloleamide, and N-benzylpalmitamide, high-dose groups of these macamides. The macamides groups received the commercial products (12 and 40 mg/kg, ig), while the control group received vehicle for 21 d. On the 14th day, the mice were given the weight-loaded swimming test. On the 21st day, the mice were sacrificed immediately after 90 min swimming, and some biochemical parameters were measured.

RESULTS AND DISCUSSION:

Compared with the control group, exhaustive swimming time was significantly prolonged in high-dose group of N-benzyloleamide (p < 0.05); the levels of lactic acid (LD), blood ammonia (BA), and lactate dehydrogenase (LDH) were significantly decreased (p < 0.05), whereas the levels of liver glycogen (LG) and non-esterified fatty acid (NEFA) were significantly increased (p < 0.05) in high-dose group of N-benzyloleamide. The malondialdehyde (MDA) contents in the brain, muscle, and liver were significantly decreased (p < 0.05), whereas superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities in the brain, muscle, and liver were significantly increased in high-dose group of N-benzyloleamide (p < 0.05).

CONCLUSION:

The results indicate that N-benzyloleamide has pharmaceutical property against exercise-induced fatigue, and this effect can be explained by the modulated energy metabolism and improved antioxidant status.

KEYWORDS:

Antioxidant; N-benzyloleamide; energy metabolism

PMID:
26453017
DOI:
10.3109/13880209.2015.1087036
[Indexed for MEDLINE]

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