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Talanta. 2015 Nov 1;144:1079-84. doi: 10.1016/j.talanta.2015.07.068. Epub 2015 Jul 29.

Development of novel enzyme potentiometric biosensor based on pH-sensitive field-effect transistors for aflatoxin B1 analysis in real samples.

Author information

1
Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150, Zabolotny Str., Kyiv 03680, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine; University Claude Bernard Lyon 1, Institute of Analytical Sciences, UMR5280 CNRS/UCBL/ENS, 5 rue de la Doua, 69100 Villeurbanne, France. Electronic address: stepurskaya@gmail.com.
2
Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150, Zabolotny Str., Kyiv 03680, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine.
3
Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine, 150, Zabolotny Str., Kyiv 03680, Ukraine.
4
University Claude Bernard Lyon 1, Institute of Analytical Sciences, UMR5280 CNRS/UCBL/ENS, 5 rue de la Doua, 69100 Villeurbanne, France.

Abstract

This study aimed at the development and optimization of a potentiometric biosensor based on pH-sensitive field-effect transistors and acetylcholinesterase for aflatoxin B1 determination in real samples. Optimal conditions for bioselective elements operation were defined and analytical characteristics of the proposed biosensor were studied. The proposed biosensor characterized high operational stability and reproducibility of signal. Selectivity of acetylcholinesterase-biosensor to aflatoxins in relation to other groups of toxic substances was analyzed. The developed biosensor was applied to the determination of aflatoxin B1 in real samples (sesame, walnut and pea).

KEYWORDS:

Acetylcholinesterase; Aflatoxin B1; Biosensor; Inhibitory analysis; Real samples; pH-sensitive field-effect transistors

PMID:
26452930
DOI:
10.1016/j.talanta.2015.07.068
[Indexed for MEDLINE]

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