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Talanta. 2015 Nov 1;144:1044-51. doi: 10.1016/j.talanta.2015.07.063. Epub 2015 Jul 26.

Development of N-ferrocenyl(benzoyl)amino-acid esters stationary phase for high performance liquid chromatography.

Author information

1
The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China.
2
Metallurgical and Chemical Department, Jiyuan Vocational and Technical College, Jiyuan 459000, PR China.
3
The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: yuajuan@zzu.edu.cn.
4
The College of Chemistry and Molecular Engineering, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: zsszz@126.com.

Abstract

A new stationary phase for high-performance liquid chromatography was prepared by covalently bonding N-ferrocenyl(benzoyl)amino-acid esters (L(1)) onto silica gel using 3-aminopropyltriethoxysilane as coupling reagent. The structure of new material was characterized by infrared spectroscopy, elemental analysis and thermogravimetric analysis. The chromatographic behavior of the phase was illustrated in reversed-phase (RP) and normal-phase (NP) modes using polycyclic aromatic hydrocarbons (PAHs), aromatics positional isomers, amines, 5-nitroimidazoles, organophosphorus pesticides and phenols as probes. Multiple mechanisms including hydrophobic, hydrogen bonding, π-π, dipole-dipole, charge-transfer and acid-base equilibrium interactions are involved. Thus, multi-interaction mechanisms and mixed-mode separation of the new phase can very likely guarantee its excellent chromatographic performance for the analysis of complex samples. The L(1) AminoSil column was successfully employed for the analysis of organophosphorus pesticides in vegetable.

KEYWORDS:

Ferrocene stationary phase; High performance liquid chromatography; Organophosphorus pesticide; Retention mechanism

PMID:
26452925
DOI:
10.1016/j.talanta.2015.07.063
[Indexed for MEDLINE]

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