Send to

Choose Destination
Elife. 2015 Oct 9;4:e08174. doi: 10.7554/eLife.08174.

Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche.

Author information

Stowers Institute for Medical Research, Kansas City, United States.
Department of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, United States.
Center for Life Sciences, College of Life Sciences, School of Medical Sciences, Tsinghua University, Beijing, China.


Adult stem cells continuously undergo self-renewal and generate differentiated cells. In the Drosophila ovary, two separate niches control germ line stem cell (GSC) self-renewal and differentiation processes. Compared to the self-renewing niche, relatively little is known about the maintenance and function of the differentiation niche. In this study, we show that the cellular redox state regulated by Wnt signaling is critical for the maintenance and function of the differentiation niche to promote GSC progeny differentiation. Defective Wnt signaling causes the loss of the differentiation niche and the upregulated BMP signaling in differentiated GSC progeny, thereby disrupting germ cell differentiation. Mechanistically, Wnt signaling controls the expression of multiple glutathione-S-transferase family genes and the cellular redox state. Finally, Wnt2 and Wnt4 function redundantly to maintain active Wnt signaling in the differentiation niche. Therefore, this study has revealed a novel strategy for Wnt signaling in regulating the cellular redox state and maintaining the differentiation niche.


D. melanogaster; Gst; Wnt signaling; developmental biology; differentiation niche; germ line stem cells; redox; stem cells

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center