Format

Send to

Choose Destination
Oncotarget. 2015 Oct 20;6(32):32586-601. doi: 10.18632/oncotarget.5309.

MicroRNA-506 suppresses tumor proliferation and metastasis in colon cancer by directly targeting the oncogene EZH2.

Author information

1
Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, 410008, PR China.
2
Department of Oncological Surgery, Affiliated Hospital of Xuzhou Medical College, 221000, PR China.
3
Department of Gastrointestinal Surgery, Third Xiangya Hospital, Central South University, 410008, PR China.
4
Department of Gastrointestinal Surgery, Guizhou Provincial People's Hospital, 550000, PR China.
5
Department of Hepatobiliary Surgery, Affiliated Hospital of Guilin Medical University, 541000, PR China.

Abstract

Increasing evidence reveals that aberrant expression of microRNA contributes to the development and progression of colon cancer, but the roles of microRNA-506 (miR-506) in colon cancer remain elusive. Here, we demonstrated that miR-506 was down-regulated in colon cancer tissue and cells and that miR-506 expression was inversely correlated with EZH2 expression, tumor size, lymph node invasion, TNM stage and metastasis. A high level of miR-506 identified patients with a favorable prognosis. In vitro and in vivo experiments confirmed that miR-506 inhibits the proliferation and metastasis of colon cancer, and a luciferase reporter assay confirmed that EZH2 is a direct and functional target of miR-506 via the 3'UTR of EZH2. The restoration of EZH2 expression partially reversed the proliferation and invasion of miR-506-overexpressing colon cancer cells. Moreover, we confirmed that the miR-506-EZH2 axis inhibits proliferation and metastasis by activating/suppressing specific downstream tumor-associated genes and the Wnt/β-catenin signaling pathway. Taking together, our study sheds light on the role of miR-506 as a suppressor for tumor growth and metastasis and raises the intriguing possibility that miR-506 may serve as a new potential marker for monitoring and treating colon cancer.

KEYWORDS:

EZH2; colon cancer; metastasis; miR-506; proliferation

PMID:
26452129
PMCID:
PMC4741714
DOI:
10.18632/oncotarget.5309
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Impact Journals, LLC Icon for PubMed Central
Loading ...
Support Center