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J Gerontol A Biol Sci Med Sci. 2016 Sep;71(9):1131-40. doi: 10.1093/gerona/glv151. Epub 2015 Oct 8.

Effect of Aging on Adipose Tissue Inflammation in the Knee Joints of F344BN Rats.

Author information

1
Aging and Metabolism Research Program, Oklahoma Medical Research Foundation. Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center.
2
Duke Molecular Physiology Institute and.
3
Duke Molecular Physiology Institute and Division of Rheumatology, Department of Medicine, Duke University School of Medicine, Durham, North Carolina.
4
Aging and Metabolism Research Program, Oklahoma Medical Research Foundation. Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center. Department of Geriatric Medicine, Reynolds Oklahoma Center on Aging, University of Oklahoma Health Sciences Center. Tim-Griffin@omrf.org.

Abstract

The infrapatellar fat pad (IFP) secretes inflammatory mediators in osteoarthritic knees, but the effect of aging on IFP inflammation is unknown. We tested the hypothesis that aging increases basal and interleukin-1β (IL-1β)-stimulated IFP inflammation in 10-, 20-, and 30-month-old male F344BN F1-hybrid rats. IFPs were cultured ex vivo for 24 hours and treated ±1ng/mL IL-1β to simulate injury-induced inflammation. IFP inflammation was evaluated by measuring secreted cytokine concentrations and by quantitative expression of immunoregulatory and pro- and anti-adipogenic genes. With age, osteoarthritis pathology increased and IFP mass decreased. Although adipocyte size did not change with age, variation in adipocyte size was positively associated with synovial thickness independent of age whereas associations with cartilage damage were age dependent. In the absence of IL-1β, aging was associated with a significant increase in IFP secretion of tumor necrosis factor α by 67% and IL-13 by 35% and a reduction in the expression of immunoregulatory M2 macrophage genes. However, following an IL-1β challenge, adipogenesis markers decreased and pro- and anti-inflammatory cytokines increased independent of age. The lone exception was leptin, which decreased >70% with age. Thus, although aging promotes osteoarthritis risk by increasing basal inflammation, our findings also revealed a potentially protective effect of aging by decreasing IL-1β-stimulated leptin production.

KEYWORDS:

Adipokines; Aging; Cytokines; Infrapatellar fat pad; Osteoarthritis

PMID:
26450946
PMCID:
PMC4978355
DOI:
10.1093/gerona/glv151
[Indexed for MEDLINE]
Free PMC Article

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