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Int J Syst Evol Microbiol. 2016 Jan;66(1):56-61. doi: 10.1099/ijsem.0.000669. Epub 2015 Oct 8.

Duganella ginsengisoli sp. nov., isolated from ginseng soil.

Author information

1
1​ Graduate School of Biotechnology, Ginseng Genetic Resource Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea.
2
2​ Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
3
1​ Graduate School of Biotechnology, Ginseng Genetic Resource Bank, College of Life Science, Kyung Hee University, Yongin, 446-701, Republic of Korea 2​ Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung-Hee University, Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.

Abstract

A Gram-stain-negative, rod-shaped bacterium, designated DCY83T, was isolated from soil of a ginseng field in Gwangju Province, Republic of Korea. Cells were motile by means of flagella. Growth occurred at 4-40 °C (optimum 30 °C), at pH 6-8 (optimum pH 7.0) and with ≤ 0.4 % NaCl. Strain DCY83T was able to produce siderophore and was positive for phosphate solubilization. Indole-3-acetic acid production was 12.9 μg ml- 1 after 3 days in culture. 16S rRNA gene sequence analysis showed that strain DCY83T belonged to the genus Duganella and was related most closely to Duganella sacchari Sac-22T (97.4 % similarity), Duganella zoogloeoides IAM 12670T (97.1 %) and Duganella radicis Sac-41T (97.1 %). The major fatty acids were C16 : 0 and summed feature 3 (containing C16 : 1ω7c and/or C16 : 1ω6c). The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The only quinone was ubiquinone 8. The genomic DNA G+C content was 55.3 mol%. DNA-DNA relatedness between strain DCY83T and D. sacchari KCTC 22381T, D. zoogloeoides JCM 20729T and D. radicis KCTC 22382T was 27.7, 22.4 and 35.5 %, respectively. On the basis of the phenotypic and genotypic analysis, DCY83T is classified as representing a novel species in the genus Duganella, for which the name Duganella ginsengisoli sp. nov. is proposed. The type strain is DCY83T ( = KCTC 42409T = JCM 30745T).

PMID:
26449233
DOI:
10.1099/ijsem.0.000669
[Indexed for MEDLINE]

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