Format

Send to

Choose Destination
Mol Cells. 2015 Oct;38(10):911-7. doi: 10.14348/molcells.2015.0215. Epub 2015 Oct 12.

The Mosquito Repellent Citronellal Directly Potentiates Drosophila TRPA1, Facilitating Feeding Suppression.

Author information

1
Samsung Biomedical Research Institute, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.
2
Department of Anatomy and Cell Biology, School of Medicine, Sungkyunkwan University, Suwon 16419, Korea.
3
Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea.
4
Department of Biological Sciences, College of Natural Sciences, University of Ulsan, Ulsan 680-749, Korea.
5
College of Life Sciences, Sejong University, Seoul 05006, Korea.

Abstract

Citronellal, a well-known plant-derived mosquito repellent, was previously reported to repel Drosophila melanogaster via olfactory pathways involving but not directly activating Transient Receptor Potential Ankyrin 1 (TRPA1). Here, we show that citronellal is a direct agonist for Drosophila and human TRPA1s (dTRPA1 and hTRPA1) as well as Anopheles gambiae TRPA1 (agTRPA1). Citronellal-induced activity is isoform-dependent for Drosophila and Anopheles gambiae TRPA1s. The recently identified dTRPA1(A) and ag-TRPA1(A) isoforms showed citronellal-provoked currents with EC50s of 1.0 B1 0.2 and 0.1 B1 0.03 mM, respectively, in Xenopus oocytes, while the sensitivities of TRPA1(B)s were much inferior to those of TRPA1(A)s. Citronellal dramatically enhanced the feeding-inhibitory effect of the TRPA1 agonist N-methylmaleimide (NMM) in Drosophila at an NMM concentration that barely repels flies. Thus, citronellal can promote feeding deterrence of fruit flies through direct action on gustatory dTRPA1, revealing the first isoform-specific function for TRPA1(A).

KEYWORDS:

Anopheles gambiae; Drosophila melanogaster; TRPA1; citronellal; human TRPA1; insect repellent

PMID:
26447139
PMCID:
PMC4625073
DOI:
10.14348/molcells.2015.0215
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Publishing M2Community Icon for PubMed Central
Loading ...
Support Center