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Gut Liver. 2016 May 23;10(3):382-90. doi: 10.5009/gnl14319.

Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome.

Author information

1
Department of Gastroenterology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China.
2
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China.

Abstract

BACKGROUND/AIMS:

Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of proteaseactivated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS.

METHODS:

Colonoscopic biopsies were performed of 38 subjects (20 with IBSdiarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining.

RESULTS:

Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only.

CONCLUSIONS:

Tryptase levels may upregulate the function of PAR- 2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS.

KEYWORDS:

Inflammation; Irritable bowel syndrome; PAR-2; Tryptases

PMID:
26446924
PMCID:
PMC4849691
DOI:
10.5009/gnl14319
[Indexed for MEDLINE]
Free PMC Article

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