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Methods Mol Biol. 2015;1340:41-52. doi: 10.1007/978-1-4939-2938-2_3.

Mesenchymal Stem Cells Derived from Human Bone Marrow.

Author information

1
Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland.
2
Cardiff University School of Biosciences, Museum Avenue, Cardiff, UK.
3
Musculoskeletal Regeneration, AO Research Institute Davos, Clavadelerstrasse 8, Davos Platz, 7270, Switzerland. martin.stoddart@aofoundation.org.

Abstract

Mesenchymal stem cells are found in a number of tissues and have the potential to differentiate into a range of mesenchymal lineages. This ready availability and multipotent character means that mesenchymal stem cells have become a focus for the field of tissue engineering, particularly for the repair of bone and cartilage. This chapter describes the isolation of mesenchymal stem cells from human bone marrow tissue, as well as expansion of the cells and characterisation of their multipotency.

KEYWORDS:

Adipogenesis; Chondrogenesis; Mesenchymal stem cell; Osteogenesis

PMID:
26445829
DOI:
10.1007/978-1-4939-2938-2_3
[Indexed for MEDLINE]

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