A Periosteum-Inspired 3D Hydrogel-Bioceramic Composite for Enhanced Bone Regeneration

Macromol Biosci. 2016 Feb;16(2):276-87. doi: 10.1002/mabi.201500258. Epub 2015 Oct 7.

Abstract

A 3D injectable hydrogel-bioceramic composite consisting of gelatin-3-(4-hydroxyphenyl) propionic acid (Gtn-HPA) and carboxymethyl cellulose-tyramine (CMC-Tyr), incorporated with fish scale-derived calcium phosphate (CaP), is developed for bone applications. The hydrogel-bioceramic composite has significantly improved the elastic modulus compared to the non-filled hydrogel, of which the addition of 10 w/v% CaP showed zero order fluorescein isothiocyanate (FITC)-dextran release profile and a significantly higher proliferation rate of encapsulated cells. All the samples promote the nucleation and growth of CaP minerals when exposed to 1× SBF. Overall, the hydrogel-bioceramic composite with 10 w/v% CaP can potentially be used as a periosteum-mimicking membrane to facilitate bone regeneration.

Keywords: biocompatibility; biomaterial; biomineralization; biopolymers; hydrogels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomimetic Materials* / chemistry
  • Biomimetic Materials* / pharmacology
  • Bone Regeneration / drug effects*
  • Bone Substitutes* / chemistry
  • Bone Substitutes* / pharmacology
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / pharmacology
  • Ceramics
  • Fishes
  • HEK293 Cells
  • Humans
  • Hydrogels* / chemistry
  • Hydrogels* / pharmacology
  • Membranes, Artificial*
  • Periosteum*

Substances

  • Bone Substitutes
  • Calcium Phosphates
  • Hydrogels
  • Membranes, Artificial
  • calcium phosphate