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Mol Ther. 2016 Feb;24(1):96-105. doi: 10.1038/mt.2015.188. Epub 2015 Oct 7.

Grapefruit-derived Nanovectors Delivering Therapeutic miR17 Through an Intranasal Route Inhibit Brain Tumor Progression.

Author information

1
Brown Cancer Center, Department of Microbiology and Immunology, University of Louisville, Louisville, Kentucky, USA.
2
Robley Rex Veterans Administration Medical Center, Louisville, Kentucky, USA.

Abstract

The lack of access to the brain is a major obstacle for central nervous system drug development. In this study, we demonstrate the capability of a grapefruit-derived nanovector (GNV) to carry miR17 for therapeutic treatment of mouse brain tumor. We show that GNVs coated with folic acid (FA-GNVs) are enhanced for targeting the GNVs to a folate receptor-positive GL-26 brain tumor. Additionally, FA-GNV-coated polyethylenimine (FA-pGNVs) not only enhance the capacity to carry RNA, but the toxicity of the polyethylenimine is eliminated by the GNVs. Intranasal administration of miR17 carried by FA-pGNVs led to rapid delivery of miR17 to the brain that was selectively taken up by GL-26 tumor cells. Mice treated intranasally with FA-pGNV/miR17 had delayed brain tumor growth. Our results demonstrate that this strategy may provide a noninvasive therapeutic approach for treating brain-related disease through intranasal delivery.

PMID:
26444082
PMCID:
PMC4754550
DOI:
10.1038/mt.2015.188
[Indexed for MEDLINE]
Free PMC Article

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