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Cell Calcium. 2015 Dec;58(6):589-97. doi: 10.1016/j.ceca.2015.09.006. Epub 2015 Sep 28.

Crosstalk between purinergic receptors and canonical signaling pathways in the mouse salivary gland.

Author information

1
Department of Neurosciences, University of Toledo Medical Center, 3000 Arlington Avenue, Toledo, OH 43614, USA.
2
Department of Neurosciences, University of Toledo Medical Center, 3000 Arlington Avenue, Toledo, OH 43614, USA. Electronic address: david.giovannucci@utoledo.edu.

Abstract

Isolated clusters of mouse parotid acinar cells in combination with live cell imaging were used to explore the crosstalk in molecular signaling between purinergic, cholinergic and adrenergic pathways that integrate to control fluid and protein secretion. This crosstalk was manifested by (1) β-adrenergic receptor activation and amplification of P2X4R evoked Ca(2+) signals, (2) β-adrenergic-induced amplification of P2X7R-evoked Ca(2+) signals and (3) muscarinic receptor induced activation of P2X7Rs via exocytotic activity. The findings from our study reveal that purinoceptor-mediated Ca(2+) signaling is modulated by crosstalk with canonical signaling pathways in parotid acinar cells. Integration of these signals are likely important for dynamic control of saliva secretion to match physiological demand in the parotid gland.

KEYWORDS:

Calcium signaling; Crosstalk; Epac; Exocytosis; Parotid; Purinergic receptor; Salivary gland; cAMP

PMID:
26443524
PMCID:
PMC4658264
DOI:
10.1016/j.ceca.2015.09.006
[Indexed for MEDLINE]
Free PMC Article

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