Format

Send to

Choose Destination
Methods Mol Biol. 2016;1338:245-59. doi: 10.1007/978-1-4939-2932-0_18.

Genome Editing in Rats Using TALE Nucleases.

Author information

1
Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.
2
ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France. laurent.tesson@univ-nantes.fr.
3
INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France. laurent.tesson@univ-nantes.fr.
4
Transgenic Rats Nantes IBiSA - Centre National de Recherche Scientifique, 44093, Nantes, France.
5
ITUN, CHU Nantes, 30 Bvd J. Monnet, 44093, Nantes, France.
6
INSERM UMR 1064, Center for Research in Transplantation and Immunology, Nantes, France.
7
INSERM U1154, CNRS UMR7196, Museum National d'Histoire Naturelle, 43 rue Cuvier, 75005, Paris, France.

Abstract

The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.

KEYWORDS:

Gene editing; Genetic engineering; Genomics; Homology-directed repair; Hprt; Knock-in; Knockout; Nonhomologous end joining; Rat model; Rosa26; TALE nucleases; TALEN; Targeted integration

PMID:
26443226
DOI:
10.1007/978-1-4939-2932-0_18
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center