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Dev Dyn. 2016 Jan;245(1):22-33. doi: 10.1002/dvdy.24355. Epub 2015 Nov 3.

Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo.

Author information

1
Department of Cell and Developmental Biology, State University of New York, Upstate Medical University, Syracuse, New York.

Abstract

BACKGROUND:

Motile cilia in the "organ of asymmetry" create directional fluid flows that are vital for left-right (LR) asymmetric patterning of vertebrate embryos. Organ function often depends on tightly regulated organ size control, but the role of organ of asymmetry size in LR patterning has remained unknown. Observations of the organ of asymmetry in the zebrafish, called Kupffer's vesicle (KV), have suggested significant variations in KV size in wild-type embryos, raising questions about the impact of KV organ size on LR patterning.

RESULTS:

To understand the relationship between organ of asymmetry size and its function, we characterized variations in KV at several developmental stages and in several different zebrafish strains. We found that the number of KV cilia and the size of the KV lumen were highly variable, whereas the length of KV cilia showed less variation. These variabilities were similar among different genetic backgrounds. By specifically modulating KV size and analyzing individual embryos, we identified a size threshold that is necessary for KV function.

CONCLUSIONS:

Together these results indicate the KV organ of asymmetry size is not tightly controlled during development, but rather must only exceed a threshold to direct robust LR patterning of the zebrafish embryo.

KEYWORDS:

cilia; congenital disease; developmental noise; left-right patterning; organ size control

PMID:
26442502
PMCID:
PMC5434515
DOI:
10.1002/dvdy.24355
[Indexed for MEDLINE]
Free PMC Article

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