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J Bacteriol. 1989 Jan;171(1):558-60.

Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon.

Author information

1
Department of Agricultural Chemistry, University of Tokyo, Japan.

Abstract

The rodA gene, which is responsible for the rod shape of Escherichia coli, was located 5 nucleotides downstream of another rod-shape-determining gene, pbpA, encoding penicillin-binding protein 2. The coding region for the RodA protein was 1,110 base pairs in length. Two plasmids, carrying a rodA-lacZ gene fusion with and without the pbpA promoter upstream of the gene fusion, were constructed. On the basis of the difference between the expression levels of the beta-galactosidase activity dependent on and independent of the pbpA promoter, we concluded that the pbpA and rodA genes constitute a single transcriptional unit called the rodA operon.

PMID:
2644207
PMCID:
PMC209621
DOI:
10.1128/jb.171.1.558-560.1989
[Indexed for MEDLINE]
Free PMC Article

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