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Exp Dermatol. 2016 Feb;25(2):113-9. doi: 10.1111/exd.12874. Epub 2016 Jan 11.

Phenotypic and functional changes in dermal primary fibroblasts isolated from intrinsically aged human skin.

Author information

1
INSERM U976, Centre de Recherche en Dermatologie, Paris, France.
2
Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
3
Centre de Recherche, Johnson & Johnson Santé Beauté France, Val de Reuil, France.
4
AP-HP, Service de Dermatologie, Hôpital Saint-Louis, Paris, France.

Abstract

Dermal fibroblasts play a key role in maintaining skin homoeostasis by synthesizing and degrading extracellular matrix components. During ageing, they are subjected to changes, such as the loss of type I collagen expression and an increased synthesis of metalloproteinase I, leading to fragmentation of collagen fibrils with consequent reduction of the mechanical tension and defects of skin wound healing. Most information about fibroblast ageing was obtained from experiments performed on replicative-senescent dermal fibroblasts in vitro. However, the senescence status of fibroblasts isolated from intrinsically aged skins and its consequences on functionality need to be deeper investigated. Herein, we studied age-related phenotypic and functional alteration of fibroblasts from 'young' (<35 years) and 'old' (>50 years) donors. Our results brought evidence of the senescent status of 'old' fibroblasts by senescence associated β-galactosidase (SA-βgal) positive staining and p16 expression. A PCR array focusing on senescence highlighted a subset of downregulated genes including cell cycle progression and ECM genes in 'old' fibroblasts as well as a subset of upregulated genes involved in senescence features. In 'old' fibroblasts, we measured a downregulation of proliferative and contractile capacities of migratory potential under PDGF stimulation and activation into myofibroblasts under TGFβ. Old fibroblasts were also more sensitive to oxidative stress than 'young' ones. Of interest, downregulation of p16 expression partially reversed the senescent phenotype of 'old' fibroblasts but failed to restore their functional properties. In conclusion, our data brought evidence of phenotypic and functional differences between fibroblasts from young and intrinsically aged skin that may contribute to the alterations observed with ageing.

KEYWORDS:

ageing; dermal fibroblasts; senescence; skin

PMID:
26441147
DOI:
10.1111/exd.12874
[Indexed for MEDLINE]

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