Nuclease stability of boron-modified nucleic acids: application to label-free mismatch detection

Org Biomol Chem. 2015 Nov 21;13(43):10604-8. doi: 10.1039/c5ob01815c.

Abstract

5'-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Pair Mismatch*
  • Base Sequence
  • Boronic Acids / chemistry
  • Boronic Acids / metabolism*
  • Cattle
  • Endonucleases / metabolism*
  • Nucleic Acids / chemistry
  • Nucleic Acids / metabolism*
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism*
  • Phosphoric Diester Hydrolases / metabolism
  • Snakes
  • Spectrometry, Fluorescence
  • Spleen / enzymology

Substances

  • Boronic Acids
  • Nucleic Acids
  • Oligonucleotides
  • Endonucleases
  • Phosphoric Diester Hydrolases