Abstract
5'-End boronic acid-modified oligonucleotides were evaluated against various nucleases at single and double stranded levels. The results show that these modifications induce a high resistance to degradation by calf-spleen and snake venom phosphodiesterases. More importantly, this eventually led to the development of a new label-free enzyme-assisted fluorescence-based method for single mismatch detection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Pair Mismatch*
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Base Sequence
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Boronic Acids / chemistry
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Boronic Acids / metabolism*
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Cattle
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Endonucleases / metabolism*
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Nucleic Acids / chemistry
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Nucleic Acids / metabolism*
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Oligonucleotides / chemistry
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Oligonucleotides / metabolism*
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Phosphoric Diester Hydrolases / metabolism
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Snakes
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Spectrometry, Fluorescence
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Spleen / enzymology
Substances
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Boronic Acids
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Nucleic Acids
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Oligonucleotides
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Endonucleases
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Phosphoric Diester Hydrolases