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Foodborne Pathog Dis. 2015 Oct;12(10):864-6. doi: 10.1089/fpd.2014.1910.

Isolation and characterization of Clostridium difficile in farm animals from slaughterhouse to retail stage in Isfahan, Iran.

Author information

1
1 Department of Research and Development, Deputy of Food and Drug, Isfahan University of Medical Sciences , Isfahan, Iran .
2
2 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences , Isfahan, Iran .
3
3 Department of Food Science and Technology, College of Food Science and Technology, Tehran Science and Research Branch, Islamic Azad University , Tehran, Iran .
4
4 Food Security Research Centre, Isfahan University of Medical Sciences , Isfahan, Iran .
5
5 Department of Pathobiology and Centre for Public Health and Zoonoses, Ontario Veterinary College, University of Guelph , Guelph, Ontario, Canada .
6
6 Department of Animal Science, Faculty of Agriculture and Natural Resources, Tehran Science and Research Branch, Islamic Azad University , Tehran, Iran .
7
7 Department of Biostatistics and Epidemiology, Behavioral Sciences Research Center, School of Public Health, Isfahan University of Medical Sciences , Isfahan, Iran .
8
8 Department of Microbiology, Islamic Azad University , Flavarjan Brach, Iran .

Abstract

To determine the prevalence of Clostridium difficile in farm animals from slaughterhouse through to retail stage, a total of 750 samples of feces, posteviscerated and washed carcass were collected from cattle, camels, goats, and sheep in Isfahan, Iran. The overall prevalence of C. difficile in feces, posteviscerated and washed carcass were 20 (13.3%), 23 (15.3%), and 11 (7.3%), respectively; while C. difficile was isolated from 79 (26.3%) retail samples. Twenty-nine (3.8%) isolates were toxigenic, with most toxigenic isolates (nā€‰=ā€‰17, 5.6%) identified from the retail stage. All toxigenic isolates harbored tcdA and tcdB; however, all were negative for cdtB. The 29 isolates were classified into 21 different ribotypes. This study revealed evidence of existence of toxigenic C. difficile in farm animal feces and meat in Iran.

PMID:
26440207
DOI:
10.1089/fpd.2014.1910
[Indexed for MEDLINE]

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