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Exp Mol Pathol. 2015 Dec;99(3):575-80. doi: 10.1016/j.yexmp.2015.09.014. Epub 2015 Oct 9.

Study of the activated macrophage transcriptome.

Author information

1
Institute for Atherosclerosis Research, Skolkovo Innovative Centre, P.O.B. #21, Moscow 121609, Russian Federation.
2
Institute for Atherosclerosis Research, Skolkovo Innovative Centre, P.O.B. #21, Moscow 121609, Russian Federation; Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Street, Moscow 125315, Russian Federation.
3
Department of Development and Regeneration, Biomedical Sciences Group, KU Leuven, Leuven, Belgium.
4
Institute for Atherosclerosis Research, Skolkovo Innovative Centre, P.O.B. #21, Moscow 121609, Russian Federation; Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, 8 Baltiyskaya Street, Moscow 125315, Russian Federation; Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russian Federation.

Abstract

Transcriptome analysis is a powerful modern tool to study possible alterations of gene expression associated with human diseases. It turns out to be especially promising for evaluation of gene expression changes in immunopathology, as immune cells have flexible gene expression patterns that can be switched in response to infection, inflammatory stimuli and exposure to various cytokines. In particular, macrophage polarization towards pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes can be successfully studied using the modern transcriptome analysis approaches. The two mostly used techniques for transcriptome analysis are microarray and next generation sequencing. In this review we will provide an overview of known gene expression changes associated with immunopathology and discuss the advantage and limitations of different methods of transcriptome analysis.

KEYWORDS:

Macrophage; Microarray; Monocyte; Next generation sequencing; Transcriptome

PMID:
26439118
DOI:
10.1016/j.yexmp.2015.09.014
[Indexed for MEDLINE]

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