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Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):E5725-33. doi: 10.1073/pnas.1513876112. Epub 2015 Oct 5.

Remodeling nuclear architecture allows efficient transport of herpesvirus capsids by diffusion.

Author information

1
Department of Molecular Biology, Princeton University, Princeton, NJ 08544; Princeton Neuroscience Institute, Princeton University, Princeton, NJ 08544;
2
Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544;
3
Princeton Neuroscience Institute, Princeton University, Princeton, NJ 08544;
4
Institute of Virology, Hannover Medical School, 30625 Hannover, Germany.
5
Department of Molecular Biology, Princeton University, Princeton, NJ 08544; Princeton Neuroscience Institute, Princeton University, Princeton, NJ 08544; lenquist@princeton.edu.

Abstract

The nuclear chromatin structure confines the movement of large macromolecular complexes to interchromatin corrals. Herpesvirus capsids of approximately 125 nm assemble in the nucleoplasm and must reach the nuclear membranes for egress. Previous studies concluded that nuclear herpesvirus capsid motility is active, directed, and based on nuclear filamentous actin, suggesting that large nuclear complexes need metabolic energy to escape nuclear entrapment. However, this hypothesis has recently been challenged. Commonly used microscopy techniques do not allow the imaging of rapid nuclear particle motility with sufficient spatiotemporal resolution. Here, we use a rotating, oblique light sheet, which we dubbed a ring-sheet, to image and track viral capsids with high temporal and spatial resolution. We do not find any evidence for directed transport. Instead, infection with different herpesviruses induced an enlargement of interchromatin domains and allowed particles to diffuse unrestricted over longer distances, thereby facilitating nuclear egress for a larger fraction of capsids.

KEYWORDS:

capsid; herpes; light sheet; nucleus; ring sheet

PMID:
26438852
PMCID:
PMC4620878
DOI:
10.1073/pnas.1513876112
[Indexed for MEDLINE]
Free PMC Article

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