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Antonie Van Leeuwenhoek. 2015 Dec;108(6):1457-1468. doi: 10.1007/s10482-015-0601-5. Epub 2015 Oct 5.

Taxonomic characterisation of Proteus terrae sp. nov., a N2O-producing, nitrate-ammonifying soil bacterium.

Author information

1
Leibniz Centre for Agricultural Landscape Research (ZALF), Institute for Landscape Biogeochemistry, Eberswalder Str. 84, 15374, Müncheberg, Germany. ubehrendt@zalf.de.
2
Leibniz Centre for Agricultural Landscape Research (ZALF), Institute for Landscape Biogeochemistry, Eberswalder Str. 84, 15374, Müncheberg, Germany.
3
Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, 38124, Braunschweig, Germany.
4
Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Central Chemical Laboratory, Müggelseedamm 301, 12587, Berlin, Germany.

Abstract

In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA-DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687(T) =DSM 29910(T) =LMG 28659(T)) is proposed.

KEYWORDS:

C/N ratio; Dissimilatory nitrate reduction to ammonium; Fen peat soil; Nitrous oxide production; Phenotypic characterisation; Phylogenetic characterization; Proteus terrae sp. nov.

PMID:
26437638
DOI:
10.1007/s10482-015-0601-5
[Indexed for MEDLINE]

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