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Trends Cell Biol. 2016 Jan;26(1):65-75. doi: 10.1016/j.tcb.2015.08.006. Epub 2015 Oct 1.

Nuclear Reprogramming by Defined Factors: Quantity Versus Quality.

Author information

1
Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
2
Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel. Electronic address: yossibug@ekmd.huji.ac.il.

Abstract

The generation of induced pluripotent stem cells (iPSCs) and directly converted cells holds great promise in regenerative medicine. However, after in-depth studies of the murine system, we know that the current methodologies to produce these cells are not ideal and mostly yield cells of poor quality that might hold a risk in therapeutic applications. In this review we address the duality found in the literature regarding the use of 'quality' as a criterion for the clinic. We discuss the elements that influence reprogramming quality, and provide evidence that safety and functionality are directly linked to cell quality. Finally, because most of the available data come from murine systems, we speculate about what aspects can be applied to human cells.

KEYWORDS:

direct conversion; epigenetics; induced pluripotent stem cells (iPSCs); nuclear reprogramming; regenerative medicine; transdifferentiation

PMID:
26437595
DOI:
10.1016/j.tcb.2015.08.006
[Indexed for MEDLINE]

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